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Image Search Results
Journal: British Journal of Pharmacology
Article Title: Nicotinic ACh receptor α7 inhibits PDGF‐induced migration of vascular smooth muscle cells by activating mitochondrial deacetylase sirtuin 3
doi: 10.1111/bph.14506
Figure Lengend Snippet: Activation of α7nAChRs inhibits the stimulation of the FAK‐Src signalling pathway induced by PDGF‐BB. (A) Immunoblotting analysis of FAK phosphorylation at Tyr397 site in primary mouse VSMCs treated with PDGF‐BB (20, 50 and 200 ng·mL−1) or PDGF‐BB (200 ng·mL−1) + PNU‐282987 (10 μM) for 72 h. *P < 0.05 versus blank, #P < 0.05 PDGF‐BB + PNU‐282985 versus PDGF‐BB. n = 6 per group. (B) Immunoblotting analysis of Src phosphorylation at Tyr416 site in primary mouse VSMCs treated with PDGF‐BB (20, 50 and 200 ng·mL−1) or PDGF‐BB (200 ng·mL−1) + PNU‐282987 (10 μM) for 72 h. *P < 0.05 versus blank, #P < 0.05 PDGF‐BB + PNU‐282985 versus PDGF‐BB. n = 6 per group. One‐way ANOVA with a post hoc Tukey's test was used for all the statistical analyses.
Article Snippet: The following antibodies were used: anti‐phosphorylated‐ http://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=2180 (p‐FAK Tyr397 , #8556, Cell Signaling Technology, 1:2000 dilution), anti‐total‐FAK (FAK, sc‐557, Santa Cruz Biotechnology, 1:2000 dilution), anti‐phosphorylated‐ http://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=2206
Techniques: Activation Assay, Western Blot, Phospho-proteomics