rabbit anti psrc tyr416 Search Results


rabbit anti phospho src family tyr416  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc rabbit anti phospho src family tyr416
Rabbit Anti Phospho Src Family Tyr416, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti phospho src tyr416 antibody  (Cell Signaling Technology Inc)
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FIGURE 6. Role of Nox4 in Ang II-induced Src and PDK-1 tyrosine phosphorylation and activation. A–G, cells were untransfected, transfected with nontargeting siRNA (Scr) or siNox4, and stimulated with Ang II (1 M) for 10 min. A and B, Src phosphorylation on tyrosine 416 <t>(P-Tyr416)</t> was detected by Western blot analysis or immunofluorescence, respectively. B, the right panel represents the semiquantification of the fluorescence intensity. C and D, PDK-1 kinase activity was determined as described in the legends to Figs. 1A and 2E, respectively. E, PDK-1 total tyrosine phosphorylation was assessed as described in the legend to Fig. 1B. F and G, PDK-1 phosphorylation on tyrosine 9 (P-Tyr9) or tyrosine 373/376 (P-Tyr373/376) was detected by direct immu- noblotting. In A and D–G, the histograms represent the ratio of the intensity of tyrosine-phosphorylated PDK-1 bands quantified by densitometry factored by the densitometric measurement of total Src, PDK-1, or actin bands. The data are expressed as percentage of control (untransfected cells without Ang II), where the ratio in the control was defined as 100%. In each panel, values are the means S.E. from three independent experi- ments. **, p 0.01 versus control; ##, p 0.01 versus untransfected cells treated with Ang II. IB, immunoblot; IP, immunoprecipitation.
Rabbit Anti Phospho Src Tyr416 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse monoclonal anti phospho tyr 416 antibody  (Cell Signaling Technology Inc)
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FIGURE 6. Role of Nox4 in Ang II-induced Src and PDK-1 tyrosine phosphorylation and activation. A–G, cells were untransfected, transfected with nontargeting siRNA (Scr) or siNox4, and stimulated with Ang II (1 M) for 10 min. A and B, Src phosphorylation on tyrosine 416 <t>(P-Tyr416)</t> was detected by Western blot analysis or immunofluorescence, respectively. B, the right panel represents the semiquantification of the fluorescence intensity. C and D, PDK-1 kinase activity was determined as described in the legends to Figs. 1A and 2E, respectively. E, PDK-1 total tyrosine phosphorylation was assessed as described in the legend to Fig. 1B. F and G, PDK-1 phosphorylation on tyrosine 9 (P-Tyr9) or tyrosine 373/376 (P-Tyr373/376) was detected by direct immu- noblotting. In A and D–G, the histograms represent the ratio of the intensity of tyrosine-phosphorylated PDK-1 bands quantified by densitometry factored by the densitometric measurement of total Src, PDK-1, or actin bands. The data are expressed as percentage of control (untransfected cells without Ang II), where the ratio in the control was defined as 100%. In each panel, values are the means S.E. from three independent experi- ments. **, p 0.01 versus control; ##, p 0.01 versus untransfected cells treated with Ang II. IB, immunoblot; IP, immunoprecipitation.
Mouse Monoclonal Anti Phospho Tyr 416 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphorylated src  (Cell Signaling Technology Inc)
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FIGURE 6. Role of Nox4 in Ang II-induced Src and PDK-1 tyrosine phosphorylation and activation. A–G, cells were untransfected, transfected with nontargeting siRNA (Scr) or siNox4, and stimulated with Ang II (1 M) for 10 min. A and B, Src phosphorylation on tyrosine 416 <t>(P-Tyr416)</t> was detected by Western blot analysis or immunofluorescence, respectively. B, the right panel represents the semiquantification of the fluorescence intensity. C and D, PDK-1 kinase activity was determined as described in the legends to Figs. 1A and 2E, respectively. E, PDK-1 total tyrosine phosphorylation was assessed as described in the legend to Fig. 1B. F and G, PDK-1 phosphorylation on tyrosine 9 (P-Tyr9) or tyrosine 373/376 (P-Tyr373/376) was detected by direct immu- noblotting. In A and D–G, the histograms represent the ratio of the intensity of tyrosine-phosphorylated PDK-1 bands quantified by densitometry factored by the densitometric measurement of total Src, PDK-1, or actin bands. The data are expressed as percentage of control (untransfected cells without Ang II), where the ratio in the control was defined as 100%. In each panel, values are the means S.E. from three independent experi- ments. **, p 0.01 versus control; ##, p 0.01 versus untransfected cells treated with Ang II. IB, immunoblot; IP, immunoprecipitation.
Phosphorylated Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit mab anti human phospho fyn  (Cell Signaling Technology Inc)
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FIGURE 6. Role of Nox4 in Ang II-induced Src and PDK-1 tyrosine phosphorylation and activation. A–G, cells were untransfected, transfected with nontargeting siRNA (Scr) or siNox4, and stimulated with Ang II (1 M) for 10 min. A and B, Src phosphorylation on tyrosine 416 <t>(P-Tyr416)</t> was detected by Western blot analysis or immunofluorescence, respectively. B, the right panel represents the semiquantification of the fluorescence intensity. C and D, PDK-1 kinase activity was determined as described in the legends to Figs. 1A and 2E, respectively. E, PDK-1 total tyrosine phosphorylation was assessed as described in the legend to Fig. 1B. F and G, PDK-1 phosphorylation on tyrosine 9 (P-Tyr9) or tyrosine 373/376 (P-Tyr373/376) was detected by direct immu- noblotting. In A and D–G, the histograms represent the ratio of the intensity of tyrosine-phosphorylated PDK-1 bands quantified by densitometry factored by the densitometric measurement of total Src, PDK-1, or actin bands. The data are expressed as percentage of control (untransfected cells without Ang II), where the ratio in the control was defined as 100%. In each panel, values are the means S.E. from three independent experi- ments. **, p 0.01 versus control; ##, p 0.01 versus untransfected cells treated with Ang II. IB, immunoblot; IP, immunoprecipitation.
Rabbit Mab Anti Human Phospho Fyn, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphor src  (Cell Signaling Technology Inc)
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FIGURE 6. Role of Nox4 in Ang II-induced Src and PDK-1 tyrosine phosphorylation and activation. A–G, cells were untransfected, transfected with nontargeting siRNA (Scr) or siNox4, and stimulated with Ang II (1 M) for 10 min. A and B, Src phosphorylation on tyrosine 416 <t>(P-Tyr416)</t> was detected by Western blot analysis or immunofluorescence, respectively. B, the right panel represents the semiquantification of the fluorescence intensity. C and D, PDK-1 kinase activity was determined as described in the legends to Figs. 1A and 2E, respectively. E, PDK-1 total tyrosine phosphorylation was assessed as described in the legend to Fig. 1B. F and G, PDK-1 phosphorylation on tyrosine 9 (P-Tyr9) or tyrosine 373/376 (P-Tyr373/376) was detected by direct immu- noblotting. In A and D–G, the histograms represent the ratio of the intensity of tyrosine-phosphorylated PDK-1 bands quantified by densitometry factored by the densitometric measurement of total Src, PDK-1, or actin bands. The data are expressed as percentage of control (untransfected cells without Ang II), where the ratio in the control was defined as 100%. In each panel, values are the means S.E. from three independent experi- ments. **, p 0.01 versus control; ##, p 0.01 versus untransfected cells treated with Ang II. IB, immunoblot; IP, immunoprecipitation.
Phosphor Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti phospho py394 lck  (Cell Signaling Technology Inc)
93
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FIGURE 6. Role of Nox4 in Ang II-induced Src and PDK-1 tyrosine phosphorylation and activation. A–G, cells were untransfected, transfected with nontargeting siRNA (Scr) or siNox4, and stimulated with Ang II (1 M) for 10 min. A and B, Src phosphorylation on tyrosine 416 <t>(P-Tyr416)</t> was detected by Western blot analysis or immunofluorescence, respectively. B, the right panel represents the semiquantification of the fluorescence intensity. C and D, PDK-1 kinase activity was determined as described in the legends to Figs. 1A and 2E, respectively. E, PDK-1 total tyrosine phosphorylation was assessed as described in the legend to Fig. 1B. F and G, PDK-1 phosphorylation on tyrosine 9 (P-Tyr9) or tyrosine 373/376 (P-Tyr373/376) was detected by direct immu- noblotting. In A and D–G, the histograms represent the ratio of the intensity of tyrosine-phosphorylated PDK-1 bands quantified by densitometry factored by the densitometric measurement of total Src, PDK-1, or actin bands. The data are expressed as percentage of control (untransfected cells without Ang II), where the ratio in the control was defined as 100%. In each panel, values are the means S.E. from three independent experi- ments. **, p 0.01 versus control; ##, p 0.01 versus untransfected cells treated with Ang II. IB, immunoblot; IP, immunoprecipitation.
Anti Phospho Py394 Lck, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti phospho src y416  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc rabbit anti phospho src y416
FIGURE 6. Role of Nox4 in Ang II-induced Src and PDK-1 tyrosine phosphorylation and activation. A–G, cells were untransfected, transfected with nontargeting siRNA (Scr) or siNox4, and stimulated with Ang II (1 M) for 10 min. A and B, Src phosphorylation on tyrosine 416 <t>(P-Tyr416)</t> was detected by Western blot analysis or immunofluorescence, respectively. B, the right panel represents the semiquantification of the fluorescence intensity. C and D, PDK-1 kinase activity was determined as described in the legends to Figs. 1A and 2E, respectively. E, PDK-1 total tyrosine phosphorylation was assessed as described in the legend to Fig. 1B. F and G, PDK-1 phosphorylation on tyrosine 9 (P-Tyr9) or tyrosine 373/376 (P-Tyr373/376) was detected by direct immu- noblotting. In A and D–G, the histograms represent the ratio of the intensity of tyrosine-phosphorylated PDK-1 bands quantified by densitometry factored by the densitometric measurement of total Src, PDK-1, or actin bands. The data are expressed as percentage of control (untransfected cells without Ang II), where the ratio in the control was defined as 100%. In each panel, values are the means S.E. from three independent experi- ments. **, p 0.01 versus control; ##, p 0.01 versus untransfected cells treated with Ang II. IB, immunoblot; IP, immunoprecipitation.
Rabbit Anti Phospho Src Y416, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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src antibody sampler kit  (Cell Signaling Technology Inc)
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FIGURE 6. Role of Nox4 in Ang II-induced Src and PDK-1 tyrosine phosphorylation and activation. A–G, cells were untransfected, transfected with nontargeting siRNA (Scr) or siNox4, and stimulated with Ang II (1 M) for 10 min. A and B, Src phosphorylation on tyrosine 416 <t>(P-Tyr416)</t> was detected by Western blot analysis or immunofluorescence, respectively. B, the right panel represents the semiquantification of the fluorescence intensity. C and D, PDK-1 kinase activity was determined as described in the legends to Figs. 1A and 2E, respectively. E, PDK-1 total tyrosine phosphorylation was assessed as described in the legend to Fig. 1B. F and G, PDK-1 phosphorylation on tyrosine 9 (P-Tyr9) or tyrosine 373/376 (P-Tyr373/376) was detected by direct immu- noblotting. In A and D–G, the histograms represent the ratio of the intensity of tyrosine-phosphorylated PDK-1 bands quantified by densitometry factored by the densitometric measurement of total Src, PDK-1, or actin bands. The data are expressed as percentage of control (untransfected cells without Ang II), where the ratio in the control was defined as 100%. In each panel, values are the means S.E. from three independent experi- ments. **, p 0.01 versus control; ##, p 0.01 versus untransfected cells treated with Ang II. IB, immunoblot; IP, immunoprecipitation.
Src Antibody Sampler Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti phospho src tyr416  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc rabbit anti phospho src tyr416
FIGURE 6. Role of Nox4 in Ang II-induced Src and PDK-1 tyrosine phosphorylation and activation. A–G, cells were untransfected, transfected with nontargeting siRNA (Scr) or siNox4, and stimulated with Ang II (1 M) for 10 min. A and B, Src phosphorylation on tyrosine 416 <t>(P-Tyr416)</t> was detected by Western blot analysis or immunofluorescence, respectively. B, the right panel represents the semiquantification of the fluorescence intensity. C and D, PDK-1 kinase activity was determined as described in the legends to Figs. 1A and 2E, respectively. E, PDK-1 total tyrosine phosphorylation was assessed as described in the legend to Fig. 1B. F and G, PDK-1 phosphorylation on tyrosine 9 (P-Tyr9) or tyrosine 373/376 (P-Tyr373/376) was detected by direct immu- noblotting. In A and D–G, the histograms represent the ratio of the intensity of tyrosine-phosphorylated PDK-1 bands quantified by densitometry factored by the densitometric measurement of total Src, PDK-1, or actin bands. The data are expressed as percentage of control (untransfected cells without Ang II), where the ratio in the control was defined as 100%. In each panel, values are the means S.E. from three independent experi- ments. **, p 0.01 versus control; ##, p 0.01 versus untransfected cells treated with Ang II. IB, immunoblot; IP, immunoprecipitation.
Rabbit Anti Phospho Src Tyr416, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit monoclonal anti phosphorylated  (Cell Signaling Technology Inc)
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FIGURE 6. Role of Nox4 in Ang II-induced Src and PDK-1 tyrosine phosphorylation and activation. A–G, cells were untransfected, transfected with nontargeting siRNA (Scr) or siNox4, and stimulated with Ang II (1 M) for 10 min. A and B, Src phosphorylation on tyrosine 416 <t>(P-Tyr416)</t> was detected by Western blot analysis or immunofluorescence, respectively. B, the right panel represents the semiquantification of the fluorescence intensity. C and D, PDK-1 kinase activity was determined as described in the legends to Figs. 1A and 2E, respectively. E, PDK-1 total tyrosine phosphorylation was assessed as described in the legend to Fig. 1B. F and G, PDK-1 phosphorylation on tyrosine 9 (P-Tyr9) or tyrosine 373/376 (P-Tyr373/376) was detected by direct immu- noblotting. In A and D–G, the histograms represent the ratio of the intensity of tyrosine-phosphorylated PDK-1 bands quantified by densitometry factored by the densitometric measurement of total Src, PDK-1, or actin bands. The data are expressed as percentage of control (untransfected cells without Ang II), where the ratio in the control was defined as 100%. In each panel, values are the means S.E. from three independent experi- ments. **, p 0.01 versus control; ##, p 0.01 versus untransfected cells treated with Ang II. IB, immunoblot; IP, immunoprecipitation.
Rabbit Monoclonal Anti Phosphorylated, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti phospho src  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc anti phospho src
FIGURE 6. Role of Nox4 in Ang II-induced Src and PDK-1 tyrosine phosphorylation and activation. A–G, cells were untransfected, transfected with nontargeting siRNA (Scr) or siNox4, and stimulated with Ang II (1 M) for 10 min. A and B, Src phosphorylation on tyrosine 416 <t>(P-Tyr416)</t> was detected by Western blot analysis or immunofluorescence, respectively. B, the right panel represents the semiquantification of the fluorescence intensity. C and D, PDK-1 kinase activity was determined as described in the legends to Figs. 1A and 2E, respectively. E, PDK-1 total tyrosine phosphorylation was assessed as described in the legend to Fig. 1B. F and G, PDK-1 phosphorylation on tyrosine 9 (P-Tyr9) or tyrosine 373/376 (P-Tyr373/376) was detected by direct immu- noblotting. In A and D–G, the histograms represent the ratio of the intensity of tyrosine-phosphorylated PDK-1 bands quantified by densitometry factored by the densitometric measurement of total Src, PDK-1, or actin bands. The data are expressed as percentage of control (untransfected cells without Ang II), where the ratio in the control was defined as 100%. In each panel, values are the means S.E. from three independent experi- ments. **, p 0.01 versus control; ##, p 0.01 versus untransfected cells treated with Ang II. IB, immunoblot; IP, immunoprecipitation.
Anti Phospho Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIGURE 6. Role of Nox4 in Ang II-induced Src and PDK-1 tyrosine phosphorylation and activation. A–G, cells were untransfected, transfected with nontargeting siRNA (Scr) or siNox4, and stimulated with Ang II (1 M) for 10 min. A and B, Src phosphorylation on tyrosine 416 (P-Tyr416) was detected by Western blot analysis or immunofluorescence, respectively. B, the right panel represents the semiquantification of the fluorescence intensity. C and D, PDK-1 kinase activity was determined as described in the legends to Figs. 1A and 2E, respectively. E, PDK-1 total tyrosine phosphorylation was assessed as described in the legend to Fig. 1B. F and G, PDK-1 phosphorylation on tyrosine 9 (P-Tyr9) or tyrosine 373/376 (P-Tyr373/376) was detected by direct immu- noblotting. In A and D–G, the histograms represent the ratio of the intensity of tyrosine-phosphorylated PDK-1 bands quantified by densitometry factored by the densitometric measurement of total Src, PDK-1, or actin bands. The data are expressed as percentage of control (untransfected cells without Ang II), where the ratio in the control was defined as 100%. In each panel, values are the means S.E. from three independent experi- ments. **, p 0.01 versus control; ##, p 0.01 versus untransfected cells treated with Ang II. IB, immunoblot; IP, immunoprecipitation.

Journal: Journal of Biological Chemistry

Article Title: Nox4 NAD(P)H Oxidase Mediates Src-dependent Tyrosine Phosphorylation of PDK-1 in Response to Angiotensin II

doi: 10.1074/jbc.m803964200

Figure Lengend Snippet: FIGURE 6. Role of Nox4 in Ang II-induced Src and PDK-1 tyrosine phosphorylation and activation. A–G, cells were untransfected, transfected with nontargeting siRNA (Scr) or siNox4, and stimulated with Ang II (1 M) for 10 min. A and B, Src phosphorylation on tyrosine 416 (P-Tyr416) was detected by Western blot analysis or immunofluorescence, respectively. B, the right panel represents the semiquantification of the fluorescence intensity. C and D, PDK-1 kinase activity was determined as described in the legends to Figs. 1A and 2E, respectively. E, PDK-1 total tyrosine phosphorylation was assessed as described in the legend to Fig. 1B. F and G, PDK-1 phosphorylation on tyrosine 9 (P-Tyr9) or tyrosine 373/376 (P-Tyr373/376) was detected by direct immu- noblotting. In A and D–G, the histograms represent the ratio of the intensity of tyrosine-phosphorylated PDK-1 bands quantified by densitometry factored by the densitometric measurement of total Src, PDK-1, or actin bands. The data are expressed as percentage of control (untransfected cells without Ang II), where the ratio in the control was defined as 100%. In each panel, values are the means S.E. from three independent experi- ments. **, p 0.01 versus control; ##, p 0.01 versus untransfected cells treated with Ang II. IB, immunoblot; IP, immunoprecipitation.

Article Snippet: Themembranes were blocked with 5% low fat milk in Tris-buffered saline and then incubated with a rabbit polyclonal Nox4 antibody (catalog number ab41886; Abcam) (dilution 1:500), a rabbit polyclonal anti-phospho-PDK-1 (Tyr9) (catalog number PP1431; ECM Biosciences) (1:1,000), a rabbit polyclonal anti-phospho-PDK-1 (Tyr373/376) (catalog number 1901-1; Epitomics Inc.) (1:1,000), a rabbit anti-phospho-Src (Tyr416) antibody or a rabbit poly- 24062 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME 283 • NUMBER 35 • AUGUST 29, 2008 clonal anti-Src (catalog numbers 2101 and 2108; Cell Signaling Technology Inc.) (1:1,000), a rabbit polyclonal anti-p70S6K (catalog number 9202; Cell Signaling Technology Inc.) (1:1,000), a rabbit polyclonal anti-Pyk-2 (catalog number 07-437; Upstate/ Millipore) (1:500), a rabbit polyclonal anti-fibronectin antibody (catalog number F3648; Sigma) (1:2,500), or a mouse mono- Image/ImageJ software, as described (23).

Techniques: Phospho-proteomics, Activation Assay, Transfection, Western Blot, Immunofluorescence, Fluorescence, Activity Assay, Control, Immunoprecipitation